Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | optimum activity at 6 mM ascorbate | Thermus thermophilus | |
ascorbate | optimum activity at 6 mM ascorbate | Sus scrofa | |
ascorbate | optimum activity at 6 mM ascorbate | Saccharomyces cerevisiae | |
L-cysteine | optimum activity at 6 mM L-cysteine | Thermus thermophilus | |
L-cysteine | optimum activity at 6 mM L-cysteine | Sus scrofa | |
L-cysteine | optimum activity at 6 mM L-cysteine | Saccharomyces cerevisiae | |
NaCl | optimum activity at 100 mM NaCl | Thermus thermophilus | |
NaCl | optimum activity at 100 mM NaCl | Sus scrofa | |
NaCl | optimum activity at 100 mM NaCl | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
expressed in Escherichia coli BL21(DE3) cells | Sus scrofa |
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
myoglobin | at pH 6.5 and 30°C | Saccharomyces cerevisiae | |
0.06 | - |
Zn2+ | at pH 6.5 and 30°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Sus scrofa | 5743 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE | Thermus thermophilus |
39000 | - |
x * 39000, SDS-PAGE | Saccharomyces cerevisiae |
42000 | - |
x * 42000, SDS-PAGE | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrin IX + Fe2+ | Thermus thermophilus | - |
protoheme IX + 2 H+ | - |
? | |
protoporphyrin IX + Fe2+ | Sus scrofa | - |
protoheme IX + 2 H+ | - |
? | |
protoporphyrin IX + Fe2+ | Saccharomyces cerevisiae | - |
protoheme IX + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Sus scrofa | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA bead chromatography | Thermus thermophilus |
Ni2+-NTA bead chromatography | Sus scrofa |
Ni2+-NTA bead chromatography | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Sus scrofa | - |
Storage Stability | Organism |
---|---|
18°C, in 20 mM Tris-HCl (pH 8.0) containing 0.3 M NaCl, about 2 weeks, 42% residual activity | Saccharomyces cerevisiae |
4°C, in 20 mM Tris-HCl (pH 8.0) containing 0.3 M NaCl, 1 month, no loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hemoglobin + Zn2+ | - |
Thermus thermophilus | Zn-protoporphyrin + H+ | - |
? | |
hemoglobin + Zn2+ | - |
Sus scrofa | Zn-protoporphyrin + H+ | - |
? | |
hemoglobin + Zn2+ | - |
Saccharomyces cerevisiae | Zn-protoporphyrin + H+ | - |
? | |
mesoporphyrin IX + Fe2+ | - |
Thermus thermophilus | ? | - |
? | |
mesoporphyrin IX + Fe2+ | - |
Sus scrofa | ? | - |
? | |
mesoporphyrin IX + Fe2+ | - |
Saccharomyces cerevisiae | ? | - |
? | |
myoglobin + Zn2+ | - |
Thermus thermophilus | Zn-protoporphyrin + H+ | - |
? | |
myoglobin + Zn2+ | - |
Sus scrofa | Zn-protoporphyrin + H+ | - |
? | |
myoglobin + Zn2+ | - |
Saccharomyces cerevisiae | Zn-protoporphyrin + H+ | - |
? | |
protoporphyrin IX + Fe2+ | - |
Thermus thermophilus | protoheme IX + 2 H+ | - |
? | |
protoporphyrin IX + Fe2+ | - |
Sus scrofa | protoheme IX + 2 H+ | - |
? | |
protoporphyrin IX + Fe2+ | - |
Saccharomyces cerevisiae | protoheme IX + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Sus scrofa |
? | x * 39000, SDS-PAGE | Saccharomyces cerevisiae |
? | x * 33000, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
FeCH | - |
Thermus thermophilus |
FeCH | - |
Sus scrofa |
FeCH | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
reverse reaction | Thermus thermophilus |
5.5 | - |
reverse reaction | Sus scrofa |
5.5 | - |
reverse reaction | Saccharomyces cerevisiae |
7.5 | 8 | forward reaction | Thermus thermophilus |
7.5 | 8 | forward reaction | Sus scrofa |
7.5 | 8 | forward reaction | Saccharomyces cerevisiae |